Remember: the number is not constant across cell lines. One of the most common mistakes that everyone makes is ignoring the number of cells plated in each well. STEP ONE: Always keep an eye on the count! (Well, I learnt it the hard way and had to struggle with it for one whole month to master it! Haha!)īut trust me, once you follow all the steps to the book then I guarantee fantastic results! Below are five steps that I have found to be the most crucial (since I work mainly on cancer therapeutics this article focuses on cancer and immune cell lines of both mouse and human origin). However, it is only an easy assay to master if you perform it carefully, paying attention to every step in detail. This chromogenic assay is extremely dependable for assessing the effects of a drug on different cell lines. The MTS cell viability assay is one of the most important yet often daunting assays to perform for researchers in cancer biology, immunology, drug delivery pharmacy, etc.
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