Note - the value obtained should be significantly greater than 10,000 M1cm1. slope y-intercept Since all the samples had the same pathlength of 1.00 cm, the Beer- Lambert equation indicates that the slope of your plot of A vs concentration will be the molar absorptivity, e, of EDMAB (in Mcm 2) at the absorption maximum. Set the Minimum value to 0 and Major Interval to 5. Report below the slope and y-intercept of the best fit line to these data. From the menu bar, select Format > Axis> Y Axis and select the Scale tab. If the plot is not linear, either your absorbance values are incorrect or your spreadsheet plot is set up incorrectly. In this plot, be sure to enter the concentration values in Molarity instead of the micro-Molar values listed on the pipette in the lab window (you need to move the decimal six places to the left). Transcribed image text: Use a spreadsheet program (such as Open Office Calc or Microsoft Excel) to create an x-y plot (called a "scatter" plot in many programs) of the absorbance (y-axis) vs concentration (x-axis) of the three known standards.
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